{"id":47458,"date":"2025-06-04T14:29:13","date_gmt":"2025-06-04T12:29:13","guid":{"rendered":"https:\/\/ellipse.prbb.org\/?p=47458"},"modified":"2025-06-06T13:36:31","modified_gmt":"2025-06-06T11:36:31","slug":"un-embrio-transparent-i-complet-de-ratoli","status":"publish","type":"post","link":"https:\/\/ellipse.prbb.org\/ca\/un-embrio-transparent-i-complet-de-ratoli\/","title":{"rendered":"Un embri\u00f3 transparent i complet de ratol\u00ed"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">En aquesta imatge podem veure un tall a trav\u00e9s de l\u2019adquisici\u00f3 <strong>3D <\/strong>d\u2019un ratol\u00ed quasi <strong>acabat de n\u00e9ixer<\/strong>, netejat amb una barreja d\u2019alcohol benz\u00edlic i de benzoat de benzil (BABB), un dissolvent org\u00e0nic que es fa servir per fer <strong>transparents<\/strong> les mostres.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">El ratol\u00ed va ser tenyit amb blau de metil\u00e8 (en cian) i l\u2019autofluoresc\u00e8ncia es va mostrar en magenta. Es pot veure tot <a href=\"https:\/\/ellipse.prbb.org\/ca\/organitzar-els-embrions-la-feina-de-nkx1-2\/\" target=\"_blank\" rel=\"noreferrer noopener\">l\u2019embri\u00f3<\/a>, del cap a la cua, i hi ha <strong>v\u00e0ries estructures anat\u00f2miques visibles<\/strong>: parts del <strong>cervell<\/strong>, la medul\u00b7la espinal i el <strong>tracte digestiu<\/strong>, aix\u00ed com els \u00f2rgans interns i les <strong>extremitats<\/strong>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><strong><a rel=\"noreferrer noopener\" href=\"https:\/\/ellipse.prbb.org\/ca\/heura-cardona-embl-barcelona-magrada-investigar-com-sorganitzen-les-cellules-fins-a-aconseguir-un-esser-viu-i-funcional\/\" target=\"_blank\">Heura Cardona<\/a><\/strong> del laboratori Sharpe <a rel=\"noreferrer noopener\" href=\"https:\/\/www.embl.es\/\" target=\"_blank\">del Laboratori Europeu de Biologia Molecular \u2013 Barcelona (EMBL Barcelona)<\/a> va proporcionar la mostra, mentre que <strong>Montserrat Coll Llad\u00f3<\/strong> del <a rel=\"noreferrer noopener\" href=\"https:\/\/ellipse.prbb.org\/ca\/imatge-mesoscopica-mif\/\" target=\"_blank\">servei d\u2019imatge mesosc\u00f2pica (MIF<\/a>, per les seves sigles en angl\u00e8s) en va processar el marcatge i neteja i va obtenir les imatges.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">La Montse ens n&#8217;explica m\u00e9s.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u201cA les nostres instal\u00b7lacions, hem rebut un microscopi basat en full de llum, el <strong>LCS SPIM<\/strong> de Luxendo (Bruker), capa\u00e7 d\u2019obtenir imatges en 3D de grans mostres \u00f2pticament netes. Vam decidir superar els nostres l\u00edmits tenyint i netejant una mostra especialment gran: un <strong>ratol\u00ed quasi acabat de n\u00e9ixer de m\u00e9s de 1,5 cm de llarg<\/strong>.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">El<strong> repte<\/strong> de veritat no va ser la neteja en s\u00ed, sin\u00f3 assegurar-nos de que tant <strong>la tinci\u00f3 com la transpar\u00e8ncia \u00f2ptica fossin uniformes al llarg de tot l\u2019organisme<\/strong>. Per aconseguir aix\u00f2, vam haver de fer diferents passos de preparaci\u00f3, incloent la descalcificaci\u00f3, <a rel=\"noreferrer noopener\" href=\"https:\/\/ellipse.prbb.org\/ca\/jugant-amb-la-pigmentacio\/\" target=\"_blank\">la reducci\u00f3 del pigment<\/a> (especialment als ulls) i una permeabilitzaci\u00f3 exhaustiva. Gr\u00e0cies a aquesta estrat\u00e8gia, vam obtenir una tinci\u00f3 uniforme i claredat al llarg de tota la mostra. Una gesta bastant extraordin\u00e0ria!\u201d<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">En efecte, felicitats a la Montse i a tot l\u2019equip i gr\u00e0cies per compartir aquesta preciosa imatge.<\/p>\n\n\n\n<div style=\"height:58px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>En aquesta imatge podem veure un tall a trav\u00e9s de l\u2019adquisici\u00f3 3D d\u2019un ratol\u00ed quasi acabat de n\u00e9ixer, netejat amb una barreja d\u2019alcohol benz\u00edlic i de benzoat de benzil (BABB), un dissolvent org\u00e0nic que es fa servir per fer transparents les mostres. El ratol\u00ed va ser tenyit amb blau de metil\u00e8 (en cian) i l\u2019autofluoresc\u00e8ncia [&hellip;]<\/p>\n","protected":false},"author":34,"featured_media":46364,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[26],"tags":[279,686,323],"new-type":[40],"class_list":["post-47458","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-recerca","tag-embl-barcelona-ca","tag-embrio","tag-microscopia","new-type-foto-de-la-setmana"],"acf":[],"_links":{"self":[{"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/posts\/47458","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/users\/34"}],"replies":[{"embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/comments?post=47458"}],"version-history":[{"count":3,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/posts\/47458\/revisions"}],"predecessor-version":[{"id":48412,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/posts\/47458\/revisions\/48412"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/media\/46364"}],"wp:attachment":[{"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/media?parent=47458"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/categories?post=47458"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/tags?post=47458"},{"taxonomy":"new-type","embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/new-type?post=47458"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}