{"id":31896,"date":"2022-08-29T09:24:03","date_gmt":"2022-08-29T07:24:03","guid":{"rendered":"https:\/\/ellipse.prbb.org\/?p=31896"},"modified":"2022-08-29T09:24:27","modified_gmt":"2022-08-29T07:24:27","slug":"identificada-una-proteina-dunio-a-larn-involucrada-en-la-metastasi-del-melanoma","status":"publish","type":"post","link":"https:\/\/ellipse.prbb.org\/ca\/identificada-una-proteina-dunio-a-larn-involucrada-en-la-metastasi-del-melanoma\/","title":{"rendered":"Identificada una prote\u00efna d\u2019uni\u00f3 a l\u2019ARN involucrada en la met\u00e0stasi del melanoma"},"content":{"rendered":"\n

El Centre de Regulaci\u00f3 Gen\u00f2mica (CRG)<\/a> ha liderat una recerca<\/a> per a con\u00e8ixer les prote\u00efnes d\u2019uni\u00f3 a l\u2019ARN <\/strong>que intervenen en la met\u00e0stasi del melanoma<\/strong><\/a>, la forma m\u00e9s letal de c\u00e0ncer de pell<\/a>. L\u2019equip, format tamb\u00e9 per investigadors de la Universitat de Halle i la Universitat d\u2019Oxford, va identificar, gr\u00e0cies a la t\u00e8cnica RIC<\/strong>, 23 prote\u00efnes que es trobaven unides a l\u2019ARN en c\u00e8l\u00b7lules tumorals molt m\u00e9s que en c\u00e8l\u00b7lules normals. Entre elles, va destacar PDIA6<\/strong>, que l\u2019equip estudi\u00e0 amb m\u00e9s detall. D\u2019aquesta manera, els estudis van revelar que les c\u00e8l\u00b7lules cancer\u00edgenes<\/a> amb menor quantitat d\u2019aquesta prote\u00efna, injectades en els animals, presenten menys capacitat de propagaci\u00f3 cap als pulmons.<\/p>\n\n\n\n

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 \u201cLes prote\u00efnes d\u2019uni\u00f3 a l\u2019ARN s\u00f3n de gran inter\u00e8s terap\u00e8utic. La t\u00e8cnica emprada en aquest estudi permet mesurar la seva activitat a una escala global, cosa que no \u00e9s possible amb m\u00e8todes convencionals\u201d <\/em>
F\u00e1tima Gebauer, CRG<\/strong><\/p><\/blockquote>\n\n\n\n

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Per a dur a terme l’estudi s’han utilitzat\u00a0xenografts en models<\/strong> de ratolins.<\/strong><\/figcaption><\/figure>\n\n\n\n
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La t\u00e8cnica RIC<\/strong><\/h3>\n\n\n\n

Dissenyada l\u2019any 2012, RIC<\/strong>, de les sigles en angl\u00e8s RNA Interactome Capture<\/em><\/strong>, <\/em>\u00e9s una t\u00e8cnica que permet caracteritzar les prote\u00efnes associades a l\u2019ARN a gran escala. Per dur-la a terme cal seguir els passos seg\u00fcents:<\/p>\n\n\n\n

  1. Primer \u00e9s necessari aplicar llum ultraviolada (UV) a les c\u00e8l\u00b7lules per a enfortir la uni\u00f3 entre l\u2019ARN i les prote\u00efnes. <\/li>
  2. Posteriorment es lisen les c\u00e8l\u00b7lules per a extraure el seu contingut i s\u2019a\u00efllen els ARN mitjan\u00e7ant un Oligo(dT), una seq\u00fc\u00e8ncia de desoxitimina (la base nitrogenada complementaria a l\u2019adenina) que s\u2019uneix a la cua terminal (poliA) de l\u2019ARN. <\/li>
  3. Finalment, gr\u00e0cies a la bola magn\u00e8tica que hi ha unida a l\u2019Oligo(dT), es \u2018pesquen\u2019 amb un imant els complexos ARN-prote\u00efnes d\u2019uni\u00f3 (interactoma<\/a>) de la mostra.<\/li>
  4. Un cop obtingut l\u2019ARN i les prote\u00efnes associades, es dissocien i es pot procedir a la identificaci\u00f3 d\u2019aquestes prote\u00efnes. <\/li><\/ol>\n\n\n\n
    <\/div>\n\n\n\n
    \"\"<\/figure>\n\n\n\n
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    La investigadora principal, F\u00e1tima Gebauer<\/strong> del CRG, assegura que actualment \u201cnom\u00e9s estem veien el potencial de les prote\u00efnes d\u2019uni\u00f3 a l\u2019ARN\u201d. I espera que, en el futur, aquesta t\u00e8cnica que els permet comparar c\u00e8l\u00b7lules tumorals i no tumorals, serveixi tamb\u00e9 per a millorar les ter\u00e0pies personalitzades en c\u00e0ncer. <\/p>\n\n\n\n

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    El CRG ha liderat un estudi on, gr\u00e0cies a la t\u00e8cnica RIC, han pogut identificar la prote\u00efna d\u2019uni\u00f3 a l\u2019ARN PDIA6 com a facilitadora de la capacitat de met\u00e0stasi de les c\u00e8l\u00b7lules cancer\u00edgenes. <\/p>\n","protected":false},"author":29,"featured_media":31900,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[26],"tags":[555,312,265,329],"new-type":[35],"class_list":["post-31896","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-recerca","tag-arn","tag-cancer-ca","tag-crg-ca","tag-proteines","new-type-noticies"],"acf":[],"_links":{"self":[{"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/posts\/31896","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/users\/29"}],"replies":[{"embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/comments?post=31896"}],"version-history":[{"count":9,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/posts\/31896\/revisions"}],"predecessor-version":[{"id":31960,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/posts\/31896\/revisions\/31960"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/media\/31900"}],"wp:attachment":[{"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/media?parent=31896"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/categories?post=31896"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/tags?post=31896"},{"taxonomy":"new-type","embeddable":true,"href":"https:\/\/ellipse.prbb.org\/ca\/wp-json\/wp\/v2\/new-type?post=31896"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}